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sars cov 2 virus  (Sino Biological)


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    Structured Review

    Sino Biological sars cov 2 virus
    Sars Cov 2 Virus, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sars+cov+2+virus/SARS-CoV-2+(2019-nCoV)+Spike+Neutralizing+Antibody%2C+Mouse+Mab/pm41816983-281-12-32
    Average 95 stars, based on 85 article reviews
    sars cov 2 virus - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Virus:

    Article Title: A Truncated Multi-Thiol Aptamer-Based SARS-CoV-2 Electrochemical Biosensor: Towards Variant-Specific Point-of-Care Detection with Optimized Fabrication
    Article Snippet: .. The main target analyte was the wild-type S protein (S1+S2 ECD, His Tag) of the SARS-CoV-2 Virus (2019-nCoV, Sino Biological Europe GmbH, Eschborn, Germany). ..

    Article Title: Nano-Phase Separation and Analyte Binding in Aptasensors Investigated by Nano-IR Spectroscopy.
    Article Snippet: The PEGblocking molecule poly(ethylene glycol) methyl ether thiol 2000 (SigmaAldrich, Germany) was used with 5mg/mL concentration dissolved in the tris-HCl buffer. .. The full-length Spike (S) protein of the Omicron variant of SARS-CoV-2 virus with a molar mass of 142114g mol−1 (2019-nCoV, Sino Biological, Germany) was used as the target analyte dissolved in tris-HCl buffer. .. Two different substrates, namely gold single crystal with predominantly (111) orientation (Mateck GmbH, Germany) and poly-crystalline evaporated gold (250nm) on glass (Arrandee metal GmbH + Co. KG, Germany) were used for AFM-IR and IRRAS measurements respectively.

    Article Title: Titers of IgG, IgM, and IgA Against SARS-CoV-2 in Healthcare Workers from a General Hospital in Mexico City
    Article Snippet: RNA extraction from samples was performed using an automated nucleic acid platform (Maelstrom 9600, TANBead, Taipei, Taiwan) with a commercial extraction system (TANBead Viral Nucleic Acid Extraction Kit, Tiangen, Beijing, China), and SARS-CoV-2 detection was performed using the SARS-CoV-2 Real-Time PCR Detection Kit (Open Reading Frame (ORF1ab) and Nucleocapsid (N) genes) (Viasure, CerTest Biotec, Zaragoza, Spain), following the manufacturer’s instructions. .. IgM, IgA, and IgG immunoglobulins against recombinant proteins for the Nucleocapsid (NCP, Sino Biological 40592-V08B) and Spike-RBD of the SARS-CoV-2 virus (Sino Biological 40592-V08B) were detected in the serum and saliva samples [ ]. .. An indirect in-house ELISA system was used [ ]: 96-well plates (Immulon 2HB, flat bottom 3455 Thermo, Waltham, MA, USA) were coated with 100 μL of the NCP or Spike-RBD antigen at 0.5 μg/mL in PBS and left at 4 °C overnight.

    Article Title: Mass-Produced and High-Performance Nanowell Biosensor Fabricated via Semiconductor Manufacturing for Rapid and Accurate COVID-19 Diagnosis in the Clinical Field.
    Article Snippet: .. The inactivated SARS-CoV-2 virus (BetaCoV/Korea/ KCDC03/2020, NCCP43326), mouse-derived monoclonal antibody targeting the SARS-CoV-2 virus (40591-MM43), and the horse radish peroxidase (HRP) conjugated antibody from mouse derived monoclonal antibody (40150-D001-H) were purchased from Sino Biological US Inc. (Wayne, PA, USA). .. Details of the Advanced Science, 2026 suppliers for other viruses and bacteria are provided in Table S3 .

    Article Title: Titers of IgG, IgM, and IgA Against SARS-CoV-2 in Healthcare Workers from a General Hospital in Mexico City.
    Article Snippet: RNA extraction from samples was performed using an automated nucleic acid platform (Maelstrom 9600, TANBead, Taipei, Taiwan) with a commercial extraction system (TANBead Viral Nucleic Acid Extraction Kit, Tiangen, Beijing, China), and SARS-CoV-2 detection was performed using the SARS-CoV-2 Real-Time PCR Detection Kit (Open Reading Frame (ORF1ab) and Nucleocapsid (N) genes) (Viasure, CerTest Biotec, Zaragoza, Spain), following the manufacturer’s instructions. .. IgM, IgA, and IgG immunoglobulins against recombinant proteins for the Nucleocapsid (NCP, Sino Biological 40592-V08B) and Spike-RBD of the SARS-CoV-2 virus (Sino Biological 40592-V08B) were detected in the serum and saliva samples [16]. .. An indirect in-house ELISA system was used [13]: 96-well plates (Immulon 2HB, flat bottom 3455 Thermo, Waltham, MA, USA) were coated with 100 μL of the NCP or Spike-RBD antigen at 0.5 μg/mL in PBS and left at 4 ◦C overnight.

    Article Title: A Truncated Multi-Thiol Aptamer-Based SARS-CoV-2 Electrochemical Biosensor: Towards Variant-Specific Point-of-Care Detection with Optimized Fabrication
    Article Snippet: .. The main target analyte was the wild-type S protein (S1+S2 ECD, His Tag) of the SARS-CoV-2 Virus (2019-nCoV, Sino Biological Europe GmbH, Eschborn, Germany). ..

    Article Title: Nano‐Phase Separation and Analyte Binding in Aptasensors Investigated by Nano‐IR Spectroscopy
    Article Snippet: The PEG‐blocking molecule poly(ethylene glycol) methyl ether thiol 2000 (Sigma‐Aldrich, Germany) was used with 5mg/mL concentration dissolved in the tris‐HCl buffer. .. The full‐length Spike (S) protein of the Omicron variant of SARS‐CoV‐2 virus with a molar mass of 142114g mol −1 (2019‐nCoV, Sino Biological, Germany) was used as the target analyte dissolved in tris‐HCl buffer. .. Two different substrates, namely gold single crystal with predominantly (111) orientation (Mateck GmbH, Germany) and poly‐crystalline evaporated gold (250nm) on glass (Arrandee metal GmbH + Co. KG, Germany) were used for AFM‐IR and IRRAS measurements respectively.

    Variant Assay:

    Article Title: Nano-Phase Separation and Analyte Binding in Aptasensors Investigated by Nano-IR Spectroscopy.
    Article Snippet: The PEGblocking molecule poly(ethylene glycol) methyl ether thiol 2000 (SigmaAldrich, Germany) was used with 5mg/mL concentration dissolved in the tris-HCl buffer. .. The full-length Spike (S) protein of the Omicron variant of SARS-CoV-2 virus with a molar mass of 142114g mol−1 (2019-nCoV, Sino Biological, Germany) was used as the target analyte dissolved in tris-HCl buffer. .. Two different substrates, namely gold single crystal with predominantly (111) orientation (Mateck GmbH, Germany) and poly-crystalline evaporated gold (250nm) on glass (Arrandee metal GmbH + Co. KG, Germany) were used for AFM-IR and IRRAS measurements respectively.

    Article Title: Nano‐Phase Separation and Analyte Binding in Aptasensors Investigated by Nano‐IR Spectroscopy
    Article Snippet: The PEG‐blocking molecule poly(ethylene glycol) methyl ether thiol 2000 (Sigma‐Aldrich, Germany) was used with 5mg/mL concentration dissolved in the tris‐HCl buffer. .. The full‐length Spike (S) protein of the Omicron variant of SARS‐CoV‐2 virus with a molar mass of 142114g mol −1 (2019‐nCoV, Sino Biological, Germany) was used as the target analyte dissolved in tris‐HCl buffer. .. Two different substrates, namely gold single crystal with predominantly (111) orientation (Mateck GmbH, Germany) and poly‐crystalline evaporated gold (250nm) on glass (Arrandee metal GmbH + Co. KG, Germany) were used for AFM‐IR and IRRAS measurements respectively.

    Recombinant:

    Article Title: Titers of IgG, IgM, and IgA Against SARS-CoV-2 in Healthcare Workers from a General Hospital in Mexico City
    Article Snippet: RNA extraction from samples was performed using an automated nucleic acid platform (Maelstrom 9600, TANBead, Taipei, Taiwan) with a commercial extraction system (TANBead Viral Nucleic Acid Extraction Kit, Tiangen, Beijing, China), and SARS-CoV-2 detection was performed using the SARS-CoV-2 Real-Time PCR Detection Kit (Open Reading Frame (ORF1ab) and Nucleocapsid (N) genes) (Viasure, CerTest Biotec, Zaragoza, Spain), following the manufacturer’s instructions. .. IgM, IgA, and IgG immunoglobulins against recombinant proteins for the Nucleocapsid (NCP, Sino Biological 40592-V08B) and Spike-RBD of the SARS-CoV-2 virus (Sino Biological 40592-V08B) were detected in the serum and saliva samples [ ]. .. An indirect in-house ELISA system was used [ ]: 96-well plates (Immulon 2HB, flat bottom 3455 Thermo, Waltham, MA, USA) were coated with 100 μL of the NCP or Spike-RBD antigen at 0.5 μg/mL in PBS and left at 4 °C overnight.

    Article Title: Titers of IgG, IgM, and IgA Against SARS-CoV-2 in Healthcare Workers from a General Hospital in Mexico City.
    Article Snippet: RNA extraction from samples was performed using an automated nucleic acid platform (Maelstrom 9600, TANBead, Taipei, Taiwan) with a commercial extraction system (TANBead Viral Nucleic Acid Extraction Kit, Tiangen, Beijing, China), and SARS-CoV-2 detection was performed using the SARS-CoV-2 Real-Time PCR Detection Kit (Open Reading Frame (ORF1ab) and Nucleocapsid (N) genes) (Viasure, CerTest Biotec, Zaragoza, Spain), following the manufacturer’s instructions. .. IgM, IgA, and IgG immunoglobulins against recombinant proteins for the Nucleocapsid (NCP, Sino Biological 40592-V08B) and Spike-RBD of the SARS-CoV-2 virus (Sino Biological 40592-V08B) were detected in the serum and saliva samples [16]. .. An indirect in-house ELISA system was used [13]: 96-well plates (Immulon 2HB, flat bottom 3455 Thermo, Waltham, MA, USA) were coated with 100 μL of the NCP or Spike-RBD antigen at 0.5 μg/mL in PBS and left at 4 ◦C overnight.

    Derivative Assay:

    Article Title: Mass-Produced and High-Performance Nanowell Biosensor Fabricated via Semiconductor Manufacturing for Rapid and Accurate COVID-19 Diagnosis in the Clinical Field.
    Article Snippet: .. The inactivated SARS-CoV-2 virus (BetaCoV/Korea/ KCDC03/2020, NCCP43326), mouse-derived monoclonal antibody targeting the SARS-CoV-2 virus (40591-MM43), and the horse radish peroxidase (HRP) conjugated antibody from mouse derived monoclonal antibody (40150-D001-H) were purchased from Sino Biological US Inc. (Wayne, PA, USA). .. Details of the Advanced Science, 2026 suppliers for other viruses and bacteria are provided in Table S3 .

    other:

    Article Title: [2409.15489] An integrated evanescent-field biosensor in silicon
    Article Snippet: In order to block any exposed sensor regions not covered by protein A, we delivered a 1 mg/mL solution of bovine serum albumin (BSA, Sigma-Aldrich A7030-10G, lot SLBD9162V) in PBS for 22 minutes.



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    LUCas enables temporal barcoding assays for detecting multiple targets in a single reaction. ( A ) Schematic representation of the temporal barcoding assay, which enables differentiation between the trans -cleavage activity of one standard guide RNA (gRNA) and one photocleavable guide (pc-igRNA) in a single CRISPR reaction. ( B ) Demonstration of the LUCas assay for detecting two viruses (FluA and <t>SARS-CoV-2)</t> in a single reaction using a temporal-barcoding approach. The standard guide targets FluA (gRNA FluA), while the photocleavable guide is complementary to a segment of the SARS-CoV-2 virus genome. The curves show the mean of three replicates, and the shaded regions indicate the SEM. ( C ) The distinct temporal barcodes, based on measured rate changes (pre-stim and post-stim), allow distinguishing between the four possible outcomes in a single reaction. Error bars represent the mean ± SEM ( n = 3). ( D ) Measured cleaved reporter concentration over time of a reaction with a mixture of different target concentration ratios. Error bars represent the SEM ( n = 3). ( E ) Scatter plot of normalized slope difference \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $(R^{\prime }_{post-stim} - R^{\prime }_{pre-stim})/R^{\prime }_{pre-stim}$\end{document} , where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $R^{\prime }$\end{document} is the reaction slope measured using a linear fit, and ratio of target concentrations shows a linear relationship, as expected. This relationship can be used to quantitatively estimate the target concentration ratios of a sample, given knowledge about individual guide-dependent activity. Data points show the mean ± SEM ( n = 3).
    Sars Cov 2 Virus Production C Aethiops Kidney Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LUCas enables temporal barcoding assays for detecting multiple targets in a single reaction. ( A ) Schematic representation of the temporal barcoding assay, which enables differentiation between the trans -cleavage activity of one standard guide RNA (gRNA) and one photocleavable guide (pc-igRNA) in a single CRISPR reaction. ( B ) Demonstration of the LUCas assay for detecting two viruses (FluA and <t>SARS-CoV-2)</t> in a single reaction using a temporal-barcoding approach. The standard guide targets FluA (gRNA FluA), while the photocleavable guide is complementary to a segment of the SARS-CoV-2 virus genome. The curves show the mean of three replicates, and the shaded regions indicate the SEM. ( C ) The distinct temporal barcodes, based on measured rate changes (pre-stim and post-stim), allow distinguishing between the four possible outcomes in a single reaction. Error bars represent the mean ± SEM ( n = 3). ( D ) Measured cleaved reporter concentration over time of a reaction with a mixture of different target concentration ratios. Error bars represent the SEM ( n = 3). ( E ) Scatter plot of normalized slope difference \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $(R^{\prime }_{post-stim} - R^{\prime }_{pre-stim})/R^{\prime }_{pre-stim}$\end{document} , where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $R^{\prime }$\end{document} is the reaction slope measured using a linear fit, and ratio of target concentrations shows a linear relationship, as expected. This relationship can be used to quantitatively estimate the target concentration ratios of a sample, given knowledge about individual guide-dependent activity. Data points show the mean ± SEM ( n = 3).
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    LUCas enables temporal barcoding assays for detecting multiple targets in a single reaction. ( A ) Schematic representation of the temporal barcoding assay, which enables differentiation between the trans -cleavage activity of one standard guide RNA (gRNA) and one photocleavable guide (pc-igRNA) in a single CRISPR reaction. ( B ) Demonstration of the LUCas assay for detecting two viruses (FluA and SARS-CoV-2) in a single reaction using a temporal-barcoding approach. The standard guide targets FluA (gRNA FluA), while the photocleavable guide is complementary to a segment of the SARS-CoV-2 virus genome. The curves show the mean of three replicates, and the shaded regions indicate the SEM. ( C ) The distinct temporal barcodes, based on measured rate changes (pre-stim and post-stim), allow distinguishing between the four possible outcomes in a single reaction. Error bars represent the mean ± SEM ( n = 3). ( D ) Measured cleaved reporter concentration over time of a reaction with a mixture of different target concentration ratios. Error bars represent the SEM ( n = 3). ( E ) Scatter plot of normalized slope difference \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $(R^{\prime }_{post-stim} - R^{\prime }_{pre-stim})/R^{\prime }_{pre-stim}$\end{document} , where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $R^{\prime }$\end{document} is the reaction slope measured using a linear fit, and ratio of target concentrations shows a linear relationship, as expected. This relationship can be used to quantitatively estimate the target concentration ratios of a sample, given knowledge about individual guide-dependent activity. Data points show the mean ± SEM ( n = 3).

    Journal: Nucleic Acids Research

    Article Title: LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs

    doi: 10.1093/nar/gkag848

    Figure Lengend Snippet: LUCas enables temporal barcoding assays for detecting multiple targets in a single reaction. ( A ) Schematic representation of the temporal barcoding assay, which enables differentiation between the trans -cleavage activity of one standard guide RNA (gRNA) and one photocleavable guide (pc-igRNA) in a single CRISPR reaction. ( B ) Demonstration of the LUCas assay for detecting two viruses (FluA and SARS-CoV-2) in a single reaction using a temporal-barcoding approach. The standard guide targets FluA (gRNA FluA), while the photocleavable guide is complementary to a segment of the SARS-CoV-2 virus genome. The curves show the mean of three replicates, and the shaded regions indicate the SEM. ( C ) The distinct temporal barcodes, based on measured rate changes (pre-stim and post-stim), allow distinguishing between the four possible outcomes in a single reaction. Error bars represent the mean ± SEM ( n = 3). ( D ) Measured cleaved reporter concentration over time of a reaction with a mixture of different target concentration ratios. Error bars represent the SEM ( n = 3). ( E ) Scatter plot of normalized slope difference \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $(R^{\prime }_{post-stim} - R^{\prime }_{pre-stim})/R^{\prime }_{pre-stim}$\end{document} , where \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $R^{\prime }$\end{document} is the reaction slope measured using a linear fit, and ratio of target concentrations shows a linear relationship, as expected. This relationship can be used to quantitatively estimate the target concentration ratios of a sample, given knowledge about individual guide-dependent activity. Data points show the mean ± SEM ( n = 3).

    Article Snippet: Finally, 14 mM of magnesium acetate, 2 μ l of synthetic SARS-CoV-2 RNA virus (Twist Bioscience, Cat#102019) and 25 nM of RNP were added, resulting in a 20 μ l final reaction volume.

    Techniques: Activity Assay, CRISPR, Virus, Concentration Assay